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Product Name
RPP25 antibody
- Documents
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Description
RPP25 Rabbit Polyclonal antibody. Positive IHC detected in human small intestine tissue, human cervical cancer tissue. Positive WB detected in HeLa cells, MCF7 cells. Observed molecular weight by Western-blot: 25 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ20374 antibody; RNase P protein subunit p25 antibody; RPP25 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RPP25 recombinant protein (Accession Number: NM_017793). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114822(RPP25 antibody) at dilution of 1:300
Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using Catalog No:114822(RPP25 Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human small intestine tissue slide using Catalog No:114822(RPP25 Antibody) at dilution of 1:50 (under 40x lens)
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Background
Ribonuclease P protein subunit p25(RPP25) is a component of ribonuclease P, which is a protein complex that generates mature tRNA molecular by cleaving their 5'-ends. It belongs to histone-like Alba family, also a component of RNase MRP. This subunit binds to RNA.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"