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Product Name
RPL7 antibody
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Description
RPL7 Rabbit Polyclonal antibody. Positive IHC detected in human skin cancer tissue. Positive WB detected in HEK-293 cells, mouse kidney tissue. Positive IP detected in HEK-293 cells. Observed molecular weight by Western-blot: 29-30 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
60S ribosomal protein L7 antibody; humL7 1 antibody; ribosomal protein L7 antibody; RPL7 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RPL7 recombinant protein (Accession Number: NM_000971). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:114899(RPL7 antibody) at dilution of 1:1500
Immunohistochemical of paraffin-embedded human skin cancer using Catalog No:114899(RPL7 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human skin cancer using Catalog No:114899(RPL7 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-RPL7 (IP:Catalog No:114899, 3ug; Detection:Catalog No:114899 1:1000) with HEK-293 cells lysate 880ug.
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Background
The mammalian ribosome comprises 79 ribosomal proteins and four rRNAs, which combine in equimolar ratios to form the small (40S) and large (60S) subunits. Ribosome proteins are a direct and critical target of the PI3K pathway in promoting growth.[PMID:15289434]. RPL7 binds to G-rich structures in 28S rRNA, and plays a regulatory role in the translation apparatus, inhibits cell-free translation of mRNAs. In addition, RPL7 is suggested to have a role as an integral part of natural freeze tolerance[PMID: 18706984]. Besides, RPL7 is responsible for its normal ribosome functions including the nuclear targeting, ribosome assembly and translation[PMID:17258209].
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