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Product Name
RPL29 antibody
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Description
RPL29 Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells, HeLa cells, HUVEC cells, mouse pancreas tissue. Positive IF detected in HepG2 cells. Positive IHC detected in human breast cancer tissue, human liver cancer tissue. Observed molecular weight by Western-blot: 20-25 kDa
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Tested applications
ELISA, IHC, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
60S ribosomal protein L29 antibody; HIP antibody; HUMRPL29 antibody; ribosomal protein L29 antibody; RPL29 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RPL29 recombinant protein (Accession Number: NM_000992). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:114886(RPL29 Antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:114886(RPL29 Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using Catalog No:114886(RPL29 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:114886(RPL29 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
RPL29 represents ribosomal protein L29
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References
- Yan J, Zhang H, Liu Y. Germline deletion of huntingtin causes male infertility and arrested spermiogenesis in mice. Journal of cell science. 129(3):492-501. 2016.
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