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Product Name
RPL24 antibody
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Description
RPL24 Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells, A549 cells, HepG2 cells. Positive IP detected in A549 cells. Positive IHC detected in human placenta tissue, human kidney tissue, human liver tissue, human ovary tissue, human spleen tissue. Positive IF detected in Hela cells. Observed molecular weight by Western-blot: 21-23kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
60S ribosomal protein L24 antibody; 60S ribosomal protein L30 antibody; ribosomal protein L24 antibody; RPL24 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RPL24 recombinant protein (Accession Number: NM_000986). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:114880(RPL24 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human placenta using Catalog No:114880(RPL24 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of Hela cells, using RPL24 antibody Catalog No:114880 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-RPL24 (IP:Catalog No:114880, 3ug; Detection:Catalog No:114880 1:500) with A549 cells lysate 2800ug.
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Background
The mammalian ribosome comprises 79 ribosomal proteins and four rRNAs, which combine in equimolar ratios to form the small (40S) and large (60S) subunits. Ribosome proteins are a direct and critical target of the PI3K pathway in promoting growth.[PMID:15289434]. RPL24 is one component of the large (60S) subunits that promote the translation of uORF-containing mRNAsgene The mutation in Rpl24 result in impairment of mRNA splicing and L24 production, which in turn affects ribosome biogenesis, protein synthesis and the cell cycle. PMID:20799971]. Also RPL24 (ribosomal protein L24) is a key factor for translation reinitiation of downstream ORFs on the polycistronic cauliflower mosaic virus 35S RNA transcription unit, and may have a role in gynoecuim development.[PMID:15270688]
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"