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Product Name
RPL13 antibody
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Description
RPL13 Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue. Positive WB detected in HeLa cells, human lung tissue. Observed molecular weight by Western-blot: 24 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
60S ribosomal protein L13 antibody; BBC1 antibody; D16S444E antibody; FLJ27453 antibody; FLJ27454 antibody; ribosomal protein L13 antibody; RPL13 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RPL13 recombinant protein (Accession Number: NM_000977). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114813(RPL13 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:114813(RPL13 antibody) at dilution of 1:50 (under 10x lens)
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Background
The mammalian ribosome comprises 79 ribosomal proteins and four rRNAs, which combine in equimolar ratios to form the small (40S) and large (60S) subunits. Ribosome proteins are a direct and critical target of the PI3K pathway in promoting growth.[PMID:15289434]. RPL13 may be a plausible candidate tumor suppressor gene, as knocking down L13 expression using small interfering RNA (siRNA) resulted in drastic attenuation of cancer cell growth with significant G1 and G2/M arrest of the cell cycle[PMID:16786168].
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