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RPAP2 antibody

Cat.#: 114798

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Product Information

  • Product Name
    RPAP2 antibody
  • Documents
  • Description
    RPAP2 Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells, HeLa cells, MCF7 cells. Positive IP detected in HEK-293 cells. Observed molecular weight by Western-blot: 70kd
  • Tested applications
    ELISA, WB, IP
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    C1orf82 antibody; FLJ13150 antibody; RPAP2 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of RPAP2 recombinant protein (Accession Number: NM_024813). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:500-1:5000

  • Validations

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:114798(RPAP2 antibody) at dilution of 1:500

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:114798(RPAP2 antibody) at dilution of 1:500

    IP Result of anti-RPAP2 (IP:Catalog No:114798, 4ug; Detection:Catalog No:114798 1:1000) with HEK-293 cells lysate 2800ug.

    IP Result of anti-RPAP2 (IP:Catalog No:114798, 4ug; Detection:Catalog No:114798 1:1000) with HEK-293 cells lysate 2800ug.

  • Background
    RPAP2 belongs to the RNA Pol II-associated protein (RPAP) family, a set of proteins involved in human Pol II machinery. Recently it has been reported that RPAP2 is required for efficient transcription of snRNA genes by Pol II. siRNA-mediated knockdown of RPAP2 caused defects in snRNA gene expression. (22137580)
  • References
    • Ni Z, Xu C, Guo X. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. Nature structural & molecular biology. 21(8):686-95. 2014.
    • Zaborowska J, Baumli S, Laitem C. Herpes Simplex Virus 1 (HSV-1) ICP22 protein directly interacts with cyclin-dependent kinase (CDK)9 to inhibit RNA polymerase II transcription elongation. PloS one. 9(9):e107654. 2014.
    • Guglielmi V, Marini M, Masson ÉF. Abnormal expression of RNA polymerase II-associated proteins in muscle of patients with myofibrillar myopathies. Histopathology. 67(6):859-65. 2015.
    • Egloff S, Zaborowska J, Laitem C, Kiss T, Murphy S. Ser7 phosphorylation of the CTD recruits the RPAP2 Ser5 phosphatase to snRNA genes. Molecular cell. 45(1):111-22. 2012.
    • Ni Z, Olsen JB, Guo X. Control of the RNA polymerase II phosphorylation state in promoter regions by CTD interaction domain-containing proteins RPRD1A and RPRD1B. Transcription. 2(5):237-42. 2012.
    • Forget D, Lacombe AA, Cloutier P, Lavallée-Adam M, Blanchette M, Coulombe B. Nuclear import of RNA polymerase II is coupled with nucleocytoplasmic shuttling of the RNA polymerase II-associated protein 2. Nucleic acids research. 41(14):6881-91. 2013.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"