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Product Name
RPA2 antibody
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Description
RPA2 Rabbit Polyclonal antibody. Positive IP detected in mouse kidney tissue. Positive WB detected in A549 cells, HEK-293 cells, HeLa cells, mouse brain tissue, mouse kidney tissue, SH-SY5Y cells. Positive IF detected in HeLa cells. Positive IHC detected in human gliomas tissue. Observed molecular weight by Western-blot: 29-32 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
REPA2 antibody; Replication factor A protein 2 antibody; replication protein A2 antibody; 32kDa antibody; RF A protein 2 antibody; RP A p32 antibody; RP A p34 antibody; RPA2 antibody; RPA32 antibody; RPA34 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RPA2 recombinant protein (Accession Number: NM_002946). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
A549 cells were subjected to SDS PAGE followed by western blot with Catalog No:114794(RPA2 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:114794(RPA2 antibody) at dilution of 1:100 (under 25x lens)
IP Result of anti-RPA2 (IP:Catalog No:114794, 4ug; Detection:Catalog No:114794 1:1000) with mouse kidney tissue lysate 4000ug.
Immunofluorescent analysis of HeLa cells using Catalog No:114794(RPA2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
RPA2 represents replication protein A2, 32kDa
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References
- Jiang W, Li YQ, Liu N. 5-Azacytidine enhances the radiosensitivity of CNE2 and SUNE1 cells in vitro and in vivo possibly by altering DNA methylation. PloS one. 9(4):e93273. 2014.
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