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Product Name
ROCK2-Specific antibody
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Description
ROCK2-Specific Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, mouse brain tissue. Positive IF detected in HepG2 cells. Positive IHC detected in human colon tissue, human brain tissue. Observed molecular weight by Western-blot: 164 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
KIAA0619 antibody; p164 ROCK 2 antibody; Rho kinase 2 antibody; ROCK2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_004850). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114780(RocK2-Specific antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human colon using Catalog No:114780(RocK2-Specific antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human colon using Catalog No:114780(RocK2-Specific antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using RocK2 antibody Catalog No:114780 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
RocK2, also named as KIAA0619, p164 RocK-2 and Rho kinase 2, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. It is regulates the assembly of the actin cytoskeleton. RocK2 promotes formation of stress fibers and of focal adhesion complexes. It plays a role in smooth muscle contraction. RocK2 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein. The antibody is specific to RocK2.
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References
- Pedraza CE, Taylor C, Pereira A. Induction of oligodendrocyte differentiation and in vitro myelination by inhibition of rho-associated kinase. ASN neuro. 6(4):. 2014.
- Chen K, He H, Xie Y. miR-125a-3p and miR-483-5p promote adipogenesis via suppressing the RhoA/ROCK1/ERK1/2 pathway in multiple symmetric lipomatosis. Scientific reports. 5:11909. 2015.
- Li M, Zhuan L, Wang T. Apocynin improves erectile function in diabetic rats through regulation of NADPH oxidase expression. The journal of sexual medicine. 9(12):3041-50. 2012.
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