-
Product Name
RNMT antibody
- Documents
-
Description
RNMT Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue. Positive IF detected in Hela cells. Positive WB detected in human kidney tissue, HeLa cells, human liver tissue. Observed molecular weight by Western-blot: 55 kDa
-
Tested applications
ELISA, WB, IHC, IF
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
DKFZp686H1252 antibody; hcm1p antibody; hCMT1 antibody; hCMT1c antibody; hMet antibody; KIAA0398 antibody; MET antibody; mRNA cap methyltransferase antibody; RG7MT1 antibody; RNMT antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of RNMT recombinant protein (Accession Number: XM_047437908). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
-
Validations
human kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:114768(RNMT antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:114768(RNMT antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:114768(RNMT antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of Hela cells, using RNMT antibody Catalog No:114768 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
-
Background
RNMT, also named as mRNA cap guanine-N7 methyltransferase or KIAA0398, is a 476 amino acid protein, which contains one mRNA cap 0 methyltransferase domain and belongs to the class I-like SAM-binding methyltransferase superfamily. mRNA cap 0 methyltransferase family. RNMT localizes in the nucleus and is widely expressed in various tissues. RNMT as a mRNA-capping methyltransferase that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs and binds RNA containing 5'-terminal GpppC.
-
References
- Fustin JM, Doi M, Yamaguchi Y. RNA-methylation-dependent RNA processing controls the speed of the circadian clock. Cell. 155(4):793-806. 2013.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"