Product Name

RNF6 antibody

Documents

Description

RNF6 Rabbit Polyclonal antibody. Positive WB detected in mouse testis tissue, PC-3 cells. Positive IP detected in mouse testis tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 78 kDa

Tested applications

ELISA, WB, IF, IP

Species reactivity

Human, Mouse; other species not tested.

Alternative names

DKFZp686P0776 antibody; RNF6 antibody; SPG2 antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of RNF6 recombinant protein (Accession Number: XM_024449390). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IP: 1:200-1:2000

IF: 1:10-1:100

Validations

mouse testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:114762(RNF6 antibody) at dilution of 1:800

Immunofluorescent analysis of HepG2 cells, using RNF6 antibody Catalog No:114762 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

IP Result of anti-RNF6 (IP:Catalog No:114762, 4ug; Detection:Catalog No:114762 1:800) with mouse testis tissue lysate 4000ug.

Background

RNF6 was firstly cloned in a genetic study of chromosomal rearrangements in myeloproliferative disorders, mapping to chromosome 13q12.12 [PMID:10331950]. It containing a coiled-coil domain at the N-terminus, and a RING-H2 finger at the C-terminus which is responsible for its ubiquitin ligase activity. It also activate Inha gene expression by participating in a protein complex binding to the promoter region [PMID:11971979]. In addition, LIMK1 is a substrate of RNF6 and the RNF6-induced LIMK1 polyubiquitination is mediated via K48 of ubiquitin and leads to proteasomal degradation of the kinase [PMID:16204183].

RNF6 antibody

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