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Product Name
RNF10 antibody
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Description
RNF10 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human brain tissue. Positive IP detected in PC-3 cells. Positive WB detected in PC-3 cells, human brain tissue, Jurkat cells. Observed molecular weight by Western-blot: 90 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human; other species not tested.
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Alternative names
KIAA0262 antibody; RIE2 antibody; ring finger protein 10 antibody; RNF10 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RNF10 recombinant protein (Accession Number: NM_001330474). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:114735(RNF10 antibody) at dilution of 1:1000
IP Result of anti-RNF10 (IP:Catalog No:114735, 5ug; Detection:Catalog No:114735 1:500) with PC-3 cells lysate 2200ug.
Immunohistochemical of paraffin-embedded human brain using Catalog No:114735(RNF10 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:114735(RNF10 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using RNF10 antibody Catalog No:114735 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
RING finger (C3HC4-type zinc finger) is a variant zinc finger motif present in a new family of proteins including transcription regulators. RNF10 (RING finger protein 10), a 90-kDa protein, contains a ring finger motif which is known to be involved in protein-protein interactions. RNF10 regulates myelin-associated glycoprotein (MAG) expression and is required for myelin formation, therefore, participates in the peripheral nerve development and Schawnn cell differentiation. RNF10 is also involved in activation of p21WAF1 promoter via its activity as transcriptional factor.
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