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Product Name
RMI1 antibody
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Description
RMI1 Rabbit Polyclonal antibody. Positive IHC detected in human gliomas tissue. Positive IP detected in HeLa cells. Positive WB detected in HepG2 cells, HeLa cells, Jurkat cells. Observed molecular weight by Western-blot: 75kd
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Tested applications
ELISA, IHC, IP, WB
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Species reactivity
Human; other species not tested.
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Alternative names
BLAP75 antibody; C9orf76 antibody; FAAP75 antibody; FLJ12888 antibody; RMI1 antibody; RP11 346I8.1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RMI1 recombinant protein (Accession Number: BC064937). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:114722(RMI1 antibody) at dilution of 1:500
IP Result of anti-RMI1 (IP:Catalog No:114722, 3ug; Detection:Catalog No:114722 1:1500) with HeLa cells lysate 3800ug.
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:114722(RMI1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:114722(RMI1 antibody) at dilution of 1:100 (under 40x lens)
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Background
RMI1 is an essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. RMI1 promotes TOP3A binding to double Holliday junctions (DHJ) and hence stimulates TOP3A-mediated dissolution. RMI1 is required for BLM phosphorylation during mitosis. Within the BLM complex, RMI1 is required for BLM and TOP3A stability. (PMID: 16537486, PMID: 16595695, PMID: 15775963)
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References
- Sturzenegger A, Burdova K, Kanagaraj R. DNA2 cooperates with the WRN and BLM RecQ helicases to mediate long-range DNA end resection in human cells. The Journal of biological chemistry. 289(39):27314-26. 2014.
- Xu C, Wang Y, Wang L. Accumulation and Phosphorylation of RecQ-Mediated Genome Instability Protein 1 (RMI1) at Serine 284 and Serine 292 during Mitosis. International journal of molecular sciences. 16(11):26395-405. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"