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Product Name
RINT1 antibody
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Description
RINT1 Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells. Positive IHC detected in human breast cancer tissue, human liver cancer tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 87kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DKFZp667H2324 antibody; HsRINT 1 antibody; RAD50 interacting protein 1 antibody; RAD50 interactor 1 antibody; RINT 1 antibody; RINT1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RINT1 recombinant protein (Accession Number: NM_021930). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:114711(RINT1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:114711(RINT1 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:114711(RINT1 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
RINT-1 was first identified as a Rad50-interacting protein that participates in radiation-induced G2/M checkpoint control. It also involved in regulation of membrane traffic between the endoplasmic reticulum and the Golgi. It interacts with p130, an Rb family protein, and together they regulate the telomerase-independent telomere length. Otherwise, it interacts with Zeste white 10 with its N-terminal region throughout the cell cycle.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"