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RIC8A antibody

Cat.#: 114697

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Special Price 239.0 USD

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Product Information

  • Product Name
    RIC8A antibody
  • Documents
  • Description
    RIC8A Rabbit Polyclonal antibody. Positive WB detected in human brain tissue. Positive IHC detected in human pancreas cancer tissue. Observed molecular weight by Western-blot: 58-62kd
  • Tested applications
    ELISA, WB, IHC
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    Protein Ric 8A antibody; RIC8 antibody; RIC8A antibody; synembryn antibody; Synembryn A antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of RIC8A recombinant protein (Accession Number: BC011821). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IHC: 1:20-1:200

  • Validations

    human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:114697(RIC8A antibody) at dilution of 1:200

    human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:114697(RIC8A antibody) at dilution of 1:200

    Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:114697(RIC8A antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:114697(RIC8A antibody) at dilution of 1:50 (under 10x lens)

  • Background
    RIC8A, also named as synembryn-A, is a Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins by exchanging bound GDP for free GTP. It is involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex. RIC8A also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation.
  • References
    • Fenech C, Patrikainen L, Kerr DS. Ric-8A, a Galpha protein guanine nucleotide exchange factor potentiates taste receptor signaling. Frontiers in cellular neuroscience. 3:11. 2009.
    • Yang Y, Liu M, Li D. CYLD regulates spindle orientation by stabilizing astral microtubules and promoting dishevelled-NuMA-dynein/dynactin complex formation. Proceedings of the National Academy of Sciences of the United States of America. 111(6):2158-63. 2014.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"