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Product Name
RGS16 antibody
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Description
RGS16 Rabbit Polyclonal antibody. Positive WB detected in Y79 cells, COLO 320 cells, HepG2 cells. Positive IP detected in HepG2 cells. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 45 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human; other species not tested.
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Alternative names
A28 RGS14 antibody; A28 RGS14P antibody; hRGS r antibody; Retinal specific RGS antibody; RGS R antibody; RGS16 antibody; RGSR antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RGS16 recombinant protein (Accession Number: NM_002928). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IF: 1:10-1:100
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Validations
Y79 cells were subjected to SDS PAGE followed by western blot with Catalog No:114643(RGS16 antibody) at dilution of 1:500
IP Result of anti-RGS16 (IP:Catalog No:114643, 4ug; Detection:Catalog No:114643 1:300) with HepG2 cells lysate 3200ug.
Immunofluorescent analysis of HepG2 cells using Catalog No:114643(RGS16 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
RGS16 belongs to the 'regulator of G protein signaling' family. The RGS proteins are a family of highly diverse, multifunctional signaling proteins found in eukaryotic species ranging from yeast to mammals. It inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits. It also may play a role in regulating the kinetics of signaling in the phototransduction cascade.
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References
- Carper MB, Denvir J, Boskovic G, Primerano DA, Claudio PP. RGS16, a novel p53 and pRb cross-talk candidate inhibits migration and invasion of pancreatic cancer cells. Genes & cancer. 5(11-12):420-35. 2014.
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