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Product Name
RGN/SMP30 antibody
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Description
RGN/SMP30 Rabbit Polyclonal antibody. Positive IP detected in mouse liver tissue. Positive WB detected in mouse liver tissue, mouse small intestine tissue, rat liver tissue. Positive IHC detected in human liver tissue, human hepat℃irrhosis tissue, human kidney tissue. Observed molecular weight by Western-blot: 33 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Regucalcin antibody; RGN antibody; Senescence marker protein 30 antibody; SMP 30 antibody; SMP30 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RGN/SMP30 recombinant protein (Accession Number: NM_004683). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:114638(RGN,SMP30 antibody) at dilution of 1:1000
IP Result of anti-RGN,SMP30 (IP:Catalog No:114638, 4ug; Detection:Catalog No:114638 1:1000) with mouse liver tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human liver slide using Catalog No:114638(RGN,SMP30 Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human liver slide using Catalog No:114638(RGN,SMP30 Antibody) at dilution of 1:50
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Background
Regucalcin (RGN), also known as SMP30, is a highly conserved, calcium-binding protein, that is preferentially expressed in the liver and kidney. SMP30 is preferentially localized in hepatocytes and renal proximal tubular epithelial cells. It is known to be related to aging, hepatocyte proliferation and tumorigenesis. It may be useful for HCC serologic screening, especially for the patients with AFP Negative.
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References
- Zhao X, Cong X, Zheng L, Xu L, Yin L, Peng J. Dioscin, a natural steroid saponin, shows remarkable protective effect against acetaminophen-induced liver damage in vitro and in vivo. Toxicology letters. 214(1):69-80. 2012.
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