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Product Name
RFC4 antibody
- Documents
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Description
RFC4 Rabbit Polyclonal antibody. Positive WB detected in MCF7 cells, LNCaP cells, MCF-7 cells, Raji cells. Positive IF detected in HepG2 cells. Positive FC detected in HepG2 cells. Observed molecular weight by Western-blot: 40-45 kDa
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Tested applications
ELISA, WB, IF, FC
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Species reactivity
Human; other species not tested.
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Alternative names
A1 37 kDa subunit antibody; Activator 1 37 kDa subunit antibody; Activator 1 subunit 4 antibody; Replication factor C subunit 4 antibody; RF C 37 kDa subunit antibody; RFC37 antibody; RFC4 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RFC4 recombinant protein (Accession Number: NM_002916). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IF: 1:10-1:100
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Validations
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:114625(RFC4 antibody) at dilution of 1:500
Immunofluorescent analysis of HepG2 cells, using RFC4 antibody Catalog No:114625 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
1X10^6 HepG2 cells were stained with 0.2ug RFC4 antibody (Catalog No:114625, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
RFC4 represents replication factor C (activator 1) 4, 37kDa
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"