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Product Name
REST antibody
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Description
REST Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in L02 cells, HeLa cells, HepG2 cells. Observed molecular weight by Western-blot: 200 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human; other species not tested.
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Alternative names
NRSF antibody; REST antibody; X2 box repressor antibody; XBR antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of REST recombinant protein (Accession Number: XM_011534401). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:500-1:5000
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Validations
L02 cells were subjected to SDS PAGE followed by western blot with Catalog No:114693(REST Antibody) at dilution of 1:600
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114693(REST Antibody) at dilution of 1:1500
IP Result of anti-REST (IP:Catalog No:114693, 5ug; Detection:Catalog No:114693 1:1000) with HeLa cells lysate 2720ug.
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Background
REST also named as NRSF or X2 box repressor is a 1097 amino acid protein, which contains 9 C2H2-type zinc fingers. REST as transcriptional repressor binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Repressor element 1 (RE1) silencing transcription factor (REST), is widely expressed during embryogenesis, and plays a strategic role in terminal neuronal differentiation. The molecular weight of REST is 121 kDa, but REST was detected 200kDa by western blot in our result, which is similar to the one article (PMID: 16764822).
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