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Product Name
RDH10 antibody
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Description
RDH10 Rabbit Polyclonal antibody. Positive IF detected in RAW 264.7 cells. Positive IHC detected in human lung cancer tissue. Positive WB detected in human kidney tissue, A549 cells, HEK-293 cells, HepG2 cells, mouse kidney tissue, rat liver tissue. Positive IP detected in mouse liver tissue. Observed molecular weight by Western-blot: 39 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
RDH10 antibody; Retinol dehydrogenase 10 antibody; SDR16C4 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RDH10 recombinant protein (Accession Number: NM_172037). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
human kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:114583(RDH10 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:114583(RDH10 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:114583(RDH10 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-RDH10 (IP:Catalog No:114583, 3ug; Detection:Catalog No:114583 1:700) with mouse liver tissue lysate 4000ug.
Immunofluorescent analysis of (-20oc Ethanol) fixed RAW 264.7 cells using Catalog No:114583(RDH10 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
RDH10(Retinol dehydrogenase 10) is a strictly NAD+-dependent enzyme with multisubstrate specificity that recognizes cis-retinols as well as all-trans-retinol as substrates. RDH10-mediated oxidation of retinol plays as important a role in the control and regulation of RA production during embryogenesis as does the subsequent RALDH-mediated reaction(PMID: 22319578).
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References
- Tochitani S, Kondo S. Immunoreactivity for GABA, GAD65, GAD67 and Bestrophin-1 in the meninges and the choroid plexus: implications for non-neuronal sources for GABA in the developing mouse brain. PloS one. 8(2):e56901. 2013.
- Kuwahara A, Ozone C, Nakano T, Saito K, Eiraku M, Sasai Y. Generation of a ciliary margin-like stem cell niche from self-organizing human retinal tissue. Nature communications. 6:6286. 2015.
- DeFalco T, Potter SJ, Williams AV, Waller B, Kan MJ, Capel B. Macrophages Contribute to the Spermatogonial Niche in the Adult Testis. Cell reports. 12(7):1107-19. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"