RCC1 antibody

Cat.#: 114577

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Product Information

  • Product Name
    RCC1 antibody
  • Documents
  • Description
    RCC1 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, A431 cells, COS-7 cells, HEK-293 cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, SH-SY5Y cells. Positive IP detected in HEK-293 cells. Positive IF detected in A431 cells. Positive IHC detected in human testis tissue, human small intestine tissue. Observed molecular weight by Western-blot: 45-50 kDa
  • Tested applications
    ELISA, WB, IHC, IP, IF
  • Species reactivity
    Human,Mouse,Rat, Monkey; other species not tested.
  • Alternative names
    Cell cycle regulatory protein antibody; CHC1 antibody; RCC1 antibody; RCC1 I antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of RCC1 recombinant protein (Accession Number: NM_001048199). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:500-1:5000

    IHC: 1:20-1:200

    IF: 1:20-1:200

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114577(RCC1 antibody) at dilution of 1:1000

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114577(RCC1 antibody) at dilution of 1:1000

    IP Result of anti-RCC1 (IP:Catalog No:114577, 3ug; Detection:Catalog No:114577 1:1000) with HEK-293 cells lysate 1000ug.

    IP Result of anti-RCC1 (IP:Catalog No:114577, 3ug; Detection:Catalog No:114577 1:1000) with HEK-293 cells lysate 1000ug.

    Immunohistochemical of paraffin-embedded human testis using Catalog No:114577(RCC1 antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human testis using Catalog No:114577(RCC1 antibody) at dilution of 1:50 (under 40x lens)

    Immunofluorescent analysis of A431 cells using Catalog No:114577(RCC1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of A431 cells using Catalog No:114577(RCC1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    CHC1, also named as RCC1, promotes the exchange of ran-bound gdp by gtp. It is involved in the regulation of onset of chromosome condensation in the S-phase. Phosphorylation of RCC1 on serines located in or near its nuclear localization signal activates RCC1 to generate RanGTP on mitotic chromosomes, which is required for spindle assembly and chromosome segregation. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RCC1.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"