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Product Name
RBPJ antibody
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Description
RBPJ Rabbit Polyclonal antibody. Positive IHC detected in human pancreas cancer tissue. Positive WB detected in mouse kidney tissue, HEK-293 cells, HeLa cells, HepG2 cells, human heart tissue, human liver tissue, MCF7 cells, mouse pancreas tissue. Positive IP detected in HEK-293 cells. Observed molecular weight by Western-blot: 60 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
CBF 1 antibody; CBF1 antibody; csl antibody; IGKJRB antibody; IGKJRB1 antibody; KBF2 antibody; NY-REN-30 antibody; RBP J antibody; RBP J kappa antibody; RBP JK antibody; RBPJ antibody; RBPJK antibody; RBPSUH antibody; SUH antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RBPJ recombinant protein (Accession Number: XM_017008174). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
mouse kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:114565(RBPJ antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:114565(RBPJ antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:114565(RBPJ antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-RBPJ (IP:Catalog No:114565, 3ug; Detection:Catalog No:114565 1:1000) with HEK-293 cells lysate 4500ug.
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Background
RBPJ, also named as IGKJRB, IGKJRB1, RBPJK, RBPSUH, NY-REN-30, CBF-1 and CSL, belongs to the Su(H) family. It is a transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. RBPJ acts as a transcriptional repressor when it is not associated with Notch proteins. It is specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. RBPJ binds to the Runx3 promoter in the atrioventricular canal in vivo, and inhibition of Notch reduces RUNX3 expression in the cardiac cushion of embryonic hearts.
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References
- Fu Y, Chang AC, Fournier M, Chang L, Niessen K, Karsan A. RUNX3 maintains the mesenchymal phenotype after termination of the Notch signal. The Journal of biological chemistry. 286(13):11803-13. 2011.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"