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Product Name
RBM15 antibody
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Description
RBM15 Mouse Monoclonal antibody. Positive IF detected in Hela cells. Positive WB detected in Hela cells, HEK-293 cells, human brain tissue, human heart tissue. Positive IP detected in HEK-293 cells. Observed molecular weight by Western-blot: 107kD
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human; other species not tested.
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Alternative names
FLJ12479 antibody; FLJ21943 antibody; One twenty two protein 1 antibody; OTT antibody; OTT1 antibody; RBM15 antibody; RNA binding motif protein 15 antibody; SPEN antibody
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Isotype
Mouse IgG2a
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Preparation
This antibody was obtained by immunization of RBM15 recombinant protein (Accession Number: BC006397). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107500(RBM15 antibody) at dilution of 1:1000
Immunofluorescent analysis of Hela cells, using RBM15 antibody Catalog No: at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).
IP Result of anti-RBM15 (IP:Catalog No:107500, 3ug; Detection:Catalog No:107500 1:500) with HEK-293 cells lysate 1800ug.
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Background
RNA-binding motif protein 15 (RBM15) belongs to the SPEN protein family, which have repressor function in several signaling pathway, and may bind to RNA through interaction with spliceosome components. It was first described as a 5′ translocation partner of the MAL gene in t(1,22)(p13;q13) infant acute megakaryocytic leukemia, and it functions in murine hematopoiesis through modulating Notch-induced transcriptional activation, which play crucial roles in leukemogenesis. Decreasing RBM15 levels with RNA interference could inhibit the growth and proliferation, block the cell cycle, and induce apoptosis in CML cells.
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References
- Zhang L, Tran NT, Su H. Cross-talk between PRMT1-mediated methylation and ubiquitylation on RBM15 controls RNA splicing. eLife. 4:. 2015.
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