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Product Name
RBBP9 antibody
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Description
RBBP9 Rabbit Polyclonal antibody. Positive IP detected in mouse lung tissue. Positive WB detected in mouse testis tissue, A375 cells, human lung tissue, human spleen tissue, mouse lung tissue. Positive IHC detected in human pancreas cancer tissue. Observed molecular weight by Western-blot: 22 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
B5T overexpressed gene protein antibody; BOG antibody; Protein BOG antibody; Putative hydrolase RBBP9 antibody; RBBP 10 antibody; RBBP 9 antibody; RBBP10 antibody; RBBP9 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RBBP9 recombinant protein (Accession Number: NM_006606). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
mouse testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:114594(RBBP9 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:114594(RBBP9 antibody) at dilution of 1:50 (under 10x lens)
IP Result of anti-RBBP9 (IP:Catalog No:114594, 3ug; Detection:Catalog No:114594 1:300) with mouse lung tissue lysate 5160ug.
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Background
RBBP9, also named as BOG, RBBP10, RBBP-9, RBBP-10 and Protein BOG, belongs to the RBBP9 family. It may play a role in the transformation process due to its capacity to confer resistance to the growth-inhibitory effects of TGF-β1 through interaction with retinoblastoma and the subsequent displacement of E2F-1. RBBP9 is a tumor-associated serine hydrolase activity required for pancreatic neoplasia. It mediates suppression of TGF-β signaling is required for E-cadherin expression as loss of the serine hydrolase activity leads to a reduction in E-cadherin levels and a concomitant decrease in the integrity of tumor cell–cell junctions..RBBP9 protein levels were equivalent in paired primary tumor and nonneoplastic specimens(PMID:20080647) The genes CyFIP2 and RbBP9, which are also miss-expressed in ALS hMSC, could serve as diagnostic biomarker tools for detection of ALS in blood samples.
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References
- O'Connor MD, Wederell E, Robertson G. Retinoblastoma-binding proteins 4 and 9 are important for human pluripotent stem cell maintenance. Experimental hematology. 39(8):866-79.e1. 2011.
- Nachmany H, Wald S, Abekasis M, Bulvik S, Weil M. Two potential biomarkers identified in mesenchymal stem cells and leukocytes of patients with sporadic amyotrophic lateral sclerosis. Disease markers. 32(4):211-20. 2012.
- Shields DJ, Niessen S, Murphy EA. RBBP9: a tumor-associated serine hydrolase activity required for pancreatic neoplasia. Proceedings of the National Academy of Sciences of the United States of America. 107(5):2189-94. 2010.
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