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RAMP1 antibody

Cat.#: 114533

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Product Information

  • Product Name
    RAMP1 antibody
  • Documents
  • Description
    RAMP1 Rabbit Polyclonal antibody. Positive IHC detected in human small intestine tissue. Positive FC detected in HepG2 cells. Positive WB detected in human heart tissue. Observed molecular weight by Western-blot: 14 kDa
  • Tested applications
    ELISA, WB, IHC, FC
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    RAMP1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of RAMP1 recombinant protein (Accession Number: NM_005855). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IHC: 1:50-1:500

  • Validations

    human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:114533(RAMP1 antibody) at dilution of 1:300

    human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:114533(RAMP1 antibody) at dilution of 1:300

    Immunohistochemistry of paraffin-embedded human small intestine tissue slide using Catalog No:114533(RAMP1 Antibody) at dilution of 1:200 (under 10x lens).

    Immunohistochemistry of paraffin-embedded human small intestine tissue slide using Catalog No:114533(RAMP1 Antibody) at dilution of 1:200 (under 10x lens).

    Immunohistochemistry of paraffin-embedded human small intestine tissue slide using Catalog No:114533(RAMP1 Antibody) at dilution of 1:200 (under 40x lens).

    Immunohistochemistry of paraffin-embedded human small intestine tissue slide using Catalog No:114533(RAMP1 Antibody) at dilution of 1:200 (under 40x lens).

    1X10^6 HepG2 cells were stained with .2ug RAMP1 antibody (Catalog No:114533, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

    1X10^6 HepG2 cells were stained with .2ug RAMP1 antibody (Catalog No:114533, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

  • Background
    RAMP1 (receptor-activity-modifying protein) is a member of the RAMP family of single-transmembrane-domain proteins which consist of an N-terminal extracellular domain, a transmembrane region and a short intracellular C-terminal tail. RAMPs are required to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR, a G protein-coupled receptor, can function as either a calcitonin gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed. RAMP1 transports the CRLR to the plasma membrane and then remains associated with it to function as a terminally glycosylated CGRP receptor, while RAMP2 and RAMP3 transfer the CRLR to the cell surface to generate receptors that are preferentially selective for adrenomedullin. (PMID: 9620797; 16188935)
  • References
    • Vause CV, Durham PL. Calcitonin gene-related peptide differentially regulates gene and protein expression in trigeminal glia cells: findings from array analysis. Neuroscience letters. 473(3):163-7. 2010.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"