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Product Name
Radixin antibody
- Documents
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Description
Radixin Rabbit Polyclonal antibody. Positive FC detected in MCF-7 cells. Positive IHC detected in human pancreas cancer tissue, human liver cancer tissue, human pancreas tissue. Positive IF detected in MCF-7 cells. Positive WB detected in mouse pancreas tissue, HEK-293 cells, Y79 cells. Observed molecular weight by Western-blot: 75kd
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Tested applications
ELISA, WB, IHC, FC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DFNB24 antibody; radixin antibody; RDX antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Radixin recombinant protein (Accession Number: XM_047427390). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with Catalog No:114521(RDX antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:114521(RDX antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:114521(RDX antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of MCF-7 cells using Catalog No:114521(RDX Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
1X10^6 MCF-7 cells were stained with 0.2ug RDX antibody (Catalog No:114521, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
RDX represents radixin
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"