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Product Name
RADIL antibody
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Description
RADIL Rabbit Polyclonal antibody. Positive IF detected in Hela cells. Positive IP detected in HepG2 cells. Positive WB detected in HEK-293 cells, HeLa cells, HepG2 cells, human brain tissue, human testis tissue. Observed molecular weight by Western-blot: 117kd
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human; other species not tested.
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Alternative names
FLJ10324 antibody; FLJ60975 antibody; KIAA1849 antibody; RADIL antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RADIL recombinant protein (Accession Number: BC004919). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IF: 1:10-1:100
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:114520(RADIL antibody) at dilution of 1:500
Immunofluorescent analysis of Hela cells, using RADIL antibody Catalog No:114520 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-RADIL (IP:Catalog No:114520, 5ug; Detection:Catalog No:114520 1:800) with HepG2 cells lysate 1720ug.
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Background
Radil is a broadly expressed adapter protein that contains a RA-like domain, a PDZ domain and a DIL domain. Like RAPL and RIAM, Radil interacts preferentially with active GTP-bound Rap1, and overexpression of Radil enhances integrin-mediated adhesion. In addition, Radil knockdown inhibits cell–substrate adhesion in HT1080, AD293, and NMuMG cells and results in migration defects in SW1353 and zebrafish neural crest cells (PMID: 17704304). Radil regulates neutrophil adhesion by specifically enhancing β2-integrin activation, as well as focal adhesion kinase (FAK) and paxillin phosphorylation (PMID: 23097489).
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