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Product Name
Rad51D antibody
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Description
Rad51D Rabbit Polyclonal antibody. Positive IHC detected in human lymphoma tissue. Positive WB detected in COLO 320 cells, Jurkat cells. Observed molecular weight by Western-blot: 23-25kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
HsTRAD antibody; R51H3 antibody; RAD51 homolog D antibody; RAD51 like 3 (S. cerevisiae) antibody; RAD51 like protein 3 antibody; Rad51D antibody; RAD51L3 antibody; Trad antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Rad51D recombinant protein (Accession Number: NM_002878). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
COLO 320 cells were subjected to SDS PAGE followed by western blot with Catalog No:114517(Rad51D antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:114517(Rad51D antibody) at dilution of 1:100 (under 10x lens)
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Background
RAD51L3, also named as RAD51D , R51H3 and TRAD, belongs to the recA family and RAD51 subfamily. It is involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. The BCDX2 complex binds single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. Heterodimer of RAD51L3 is about 70 kd.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"