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Product Name
RAD1 antibody
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Description
RAD1 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells. Positive IP detected in HeLa cells. Positive IHC detected in human lymphoma tissue. Observed molecular weight by Western-blot: 32kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
HRAD1 antibody; RAD1 antibody; RAD1 homolog (S. pombe) antibody; REC1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RAD1 recombinant protein (Accession Number: NM_002853). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114509(RAD1 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:114509(RAD1 antibody) at dilution of 1:50 (under 10x lens)
IP Result of anti-RAD1 (IP:Catalog No:114509, 3ug; Detection:Catalog No:114509 1:300) with HeLa cells lysate 3200ug.
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Background
RAD1 is also named as REC1,hRAD1. The human RAD1 and HUS1 proteins associate in a complex that interacts with a highly modified form of RAD9. They concluded that these 3 proteins are central components of a DNA damage-responsive protein complex in human cells.It has 3 isoforms produced by alternative splicing.
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References
- Bozdarov J, Sherry JP, Duncker BP, Bols NC, Dixon B. The rad1 gene in Rainbow Trout (Oncorhynchus mykiss) is highly conserved and may express proteins from non-canonical spliced isoforms. Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 157(1):16-23. 2013.
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