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Product Name
Rac1 antibody
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Description
Rac1 Mouse Monoclonal antibody. Positive IP detected in HL-60 cells. Positive WB detected in HL-60 cells, human brain tissue, NIH/3T3 cells. Observed molecular weight by Western-blot: 21 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse; other species not tested.
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Alternative names
MIG5 antibody; p21 Rac1 antibody; RAC1 antibody; RAC1b antibody; Ras like protein TC25 antibody; TC 25 antibody; TC25 antibody
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Isotype
Mouse IgG2b
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Preparation
This antibody was obtained by immunization of Rac1 recombinant protein (Accession Number: NM_006908). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
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Validations
HL-60 cells were subjected to SDS PAGE followed by western blot with Catalog No:107494(RAC1 antibody) at dilution of 1:1000
IP Result of anti-RAC1 (IP:Catalog No:107494, 3ug; Detection:Catalog No:107494 1:700) with HL-60 cells lysate 4000ug.
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Background
RAC1b, also named as TC25, belongs to the small GTPase superfamily and Rho family. RAC1 is a plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, it binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles.
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References
- Zhou T, Zhang B, Wei P. Energy metabolism analysis reveals the mechanism of inhibition of breast cancer cell metastasis by PEG-modified graphene oxide nanosheets. Biomaterials. 35(37):9833-43. 2014.
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