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Product Name
RAC1/2/3 antibody
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Description
RAC1/2/3 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human brain tissue. Positive IP detected in mouse brain tissue. Positive WB detected in human brain tissue, HeLa cells, mouse brain tissue. Observed molecular weight by Western-blot: 21 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
p21 Rac3 antibody; RAC3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RAC1/2/3 recombinant protein (Accession Number: NM_005052). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:114504(RAC1/2/3 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human brain using Catalog No:114504(RAC1/2/3 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:114504(RAC1/2/3 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using RAC3 antibody Catalog No:114504 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-RAC1/2/3 (IP:Catalog No:114504, 3ug; Detection:Catalog No:114504 1:500) with mouse brain tissue lysate 2640ug.
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Background
Rac3 is a GTPase that belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. There are three Rac genes in mammals, which share over 90% protein identity but differ for their pattern of expression. Rac1 is ubiquitous, Rac2 is specific for hematopoietic cells, while Rac3 is prevalent in the developing nervous system. This antibody can recognize Rac1, Rac2, and Rac3.
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References
- Liu TQ, Wang GB, Li ZJ, Tong XD, Liu HX. Silencing of Rac3 inhibits proliferation and induces apoptosis of human lung cancer cells. Asian Pacific journal of cancer prevention : APJCP. 16(7):3061-5. 2015.
- Gest C, Joimel U, Huang L. Rac3 induces a molecular pathway triggering breast cancer cell aggressiveness: differences in MDA-MB-231 and MCF-7 breast cancer cell lines. BMC cancer. 13:63. 2013.
- Dong S, Zhao J, Wei J. F-box protein complex FBXL19 regulates TGFβ1-induced E-cadherin down-regulation by mediating Rac3 ubiquitination and degradation. Molecular cancer. 13:76. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"