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Product Name
RABEP2 antibody
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Description
RABEP2 Rabbit Polyclonal antibody. Positive IP detected in mouse liver tissue. Positive WB detected in Jurkat cells, HeLa cells, mouse liver tissue. Positive IF detected in Hela cells. Positive IHC detected in human prostate cancer tissue. Observed molecular weight by Western-blot: 59-62 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ23282 antibody; FRA antibody; Rabaptin 5beta antibody; RABEP2 antibody; RABPT5B antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RABEP2 recombinant protein (Accession Number: BC058900). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:114494(RABEP2 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:114494(RABEP2 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:114494(RABEP2 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of Hela cells, using RABEP2 antibody Catalog No:114494 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-RABEP2 (IP:Catalog No:114494, 4ug; Detection:Catalog No:114494 1:1000) with mouse liver tissue lysate 4000ug.
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Background
RABEP2, also named as RABPT5B, plays a role in membrane trafficking and in homotypic early endosome fusion. It has two isoforms with MW 59-63 kDa.
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