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Product Name
RAB9A antibody
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Description
RAB9A Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human prostate cancer tissue, human spleen tissue. Positive WB detected in HeLa cells. Observed molecular weight by Western-blot: 23 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
RAB9 antibody; RAB9A antibody; Ras related protein Rab 9A antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RAB9A recombinant protein (Accession Number: XM_047442644). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114463(RAB9A antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:114463(RAB9A antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:114463(RAB9A Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
RAB9A belongs to the small GTPase superfamily, the members of which play an important role in regulating vesicular trafficking and reside in specific intracellular compartments. RAB9A has been localized to components of the endocytic/exocytic pathway and has been implicated in recycling of membrane receptors, such as mannose 6-phosphate receptor, from early endosomes to the trans Golgi network.
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References
- Yamasaki T, Suzuki A, Shimizu T, Watarai M, Hasebe R, Horiuchi M. Characterization of intracellular localization of PrP(Sc) in prion-infected cells using a mAb that recognizes the region consisting of aa 119-127 of mouse PrP. The Journal of general virology. 93(Pt 3):668-80. 2012.
- Yamasaki T, Baron GS, Suzuki A, Hasebe R, Horiuchi M. Characterization of intracellular dynamics of inoculated PrP-res and newly generated PrP(Sc) during early stage prion infection in Neuro2a cells. Virology. 450-451:324-35. 2014.
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