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Product Name
RAB12 antibody
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Description
RAB12 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells. Positive IP detected in HeLa cells. Positive IF detected in HeLa cells. Positive IHC detected in human liver tissue. Observed molecular weight by Western-blot: 28 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human; other species not tested.
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Alternative names
FLJ45927 antibody; RAB12 antibody; Ras related protein Rab 12 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_001025300). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114412(RAB12 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human liver using Catalog No:114412(RAB12 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:114412(RAB12 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-RAB12 (IP:Catalog No:114412, 4ug; Detection:Catalog No:114412 1:500) with HeLa cells lysate 1200ug.
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:114412(RAB12 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
RAB12 belongs to the small GTPase superfamily and Rab family. RAB12 locates in Golgi apparatus membrane.
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References
- Rydell GE, Renard HF, Garcia-Castillo MD. Rab12 localizes to Shiga toxin-induced plasma membrane invaginations and controls toxin transport. Traffic (Copenhagen, Denmark). 15(7):772-87. 2014.
- Efergan A, Azouz NP, Klein O. Rab12 Regulates Retrograde Transport of Mast Cell Secretory Granules by Interacting with the RILP-Dynein Complex. Journal of immunology (Baltimore, Md. : 1950). 196(3):1091-101. 2016.
- Xu J, Fotouhi M, McPherson PS. Phosphorylation of the exchange factor DENND3 by ULK in response to starvation activates Rab12 and induces autophagy. EMBO reports. 16(6):709-18. 2015.
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