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Product Name
Pyruvate Carboxylase antibody
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Description
Pyruvate Carboxylase Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells. Positive IP detected in mouse liver tissue. Positive IHC detected in human liver tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 125-130kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
PCB antibody; pyruvate Carboxylase antibody; Pyruvic carboxylase antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Pyruvate Carboxylase recombinant protein (Accession Number: XM_017017870). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:114365(PC antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human liver using Catalog No:114365(PC antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:114365(PC antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using PC antibody Catalog No:114365 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-PC (IP:Catalog No:114365, 4ug; Detection:Catalog No:114365 1:600) with mouse liver tissue lysate 4000ug.
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Background
PC(pyruvate carboxylase) is a member of the family of biotin-dependent carboxylases and is found widely among eukaryotic tissues and in many prokaryotic species. It catalyses the ATP-dependent carboxylation of pyruvate to form oxaloacetate which may be utilised in the synthesis of glucose, fat, some amino acids or their derivatives and several neurotransmitters. Diabetes and hyperthyroidism increase the level of expression of pyruvate carboxylase in the long term, while its activity in the short term is controlled by the intramitochondrial concentrations of acetyl-CoA and pyruvate(PMID:9597748).
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References
- Pircher H, von Grafenstein S, Diener T. Identification of FAH domain-containing protein 1 (FAHD1) as oxaloacetate decarboxylase. The Journal of biological chemistry. 290(11):6755-62. 2015.
- Ulanet DB, Couto K, Jha A. Mesenchymal phenotype predisposes lung cancer cells to impaired proliferation and redox stress in response to glutaminase inhibition. PloS one. 9(12):e115144. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"