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Product Name
PYGM-Specific antibody
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Description
PYGM-Specific Rabbit Polyclonal antibody. Positive IHC detected in human skeletal muscle tissue, human liver tissue. Positive IF detected in HepG2 cells. Positive WB detected in mouse skeletal muscle tissue, HepG2 cells. Observed molecular weight by Western-blot: 97 kDa
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Tested applications
ELISA, IHC, IF, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Myophosphorylase antibody; PYGM antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_005609). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
Immunohistochemical of paraffin-embedded human skeletal muscle using Catalog No:114361(PYGM-Specific antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human skeletal muscle using Catalog No:114361(PYGM-Specific antibody) at dilution of 1:50 (under 40x lens)
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with Catalog No:114361(PYGM-Specific Antibody) at dilution of 1:1000
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:114361(PYGM-Specific Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
PYGM belongs to the glycogen phosphorylase family. Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Defects in PYGM are the cause of glycogen storage disease type 5 (GSD5) which also known as McArdle disease. The antibody is specific to PYGM.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"