-
Product Name
PYGB antibody
- Documents
-
Description
PYGB Rabbit Polyclonal antibody. Positive WB detected in A549 cells, HeLa cells, MCF7 cells. Positive IF detected in A549 cells. Observed molecular weight by Western-blot: 97-105kd
-
Tested applications
ELISA, WB, IF
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
phosphorylase antibody; glycogen; brain antibody; PYGB antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_002862). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:2000
IF: 1:20-1:200
-
Validations
A549 cells were subjected to SDS PAGE followed by western blot with Catalog No:114358(PYGB antibody) at dilution of 1:500
Immunofluorescent analysis of A549 cells using Catalog No:114358(PYGB Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
-
Background
PYGB belongs to the glycogen phosphorylase family. Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. This antibody is specific to PYGB. It has no cross reaction to PYGM and PYGL.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"