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Product Name
PSME2 antibody
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Description
PSME2 Rabbit Polyclonal antibody. Positive IP detected in MCF-7 cells. Positive WB detected in HepG2 cells, human brain tissue, human kidney tissue, human placenta tissue, MCF7 cells, mouse pancreas tissue, Raji cells. Positive IF detected in MCF-7 cells. Observed molecular weight by Western-blot: 27kd
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Tested applications
ELISA, WB, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
PA28B antibody; PA28beta antibody; PSME2 antibody; REG beta antibody; REGbeta antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PSME2 recombinant protein (Accession Number: NM_002818). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IF: 1:10-1:100
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:114247(PSME2 antibody) at dilution of 1:500
IP Result of anti-PSME2 (IP:Catalog No:114247, 4ug; Detection:Catalog No:114247 1:500) with MCF-7 cells lysate 2000ug.
Immunofluorescent analysis of MCF-7 cells using Catalog No:114247(PSME2 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
The principal function of the proteasome is targeted degradation of intracellular proteins. Activity of the 20S proteasome is controlled by regulatory complexes that bind to the ends of the cylindrical proteasome. 11S regulator (REG or PA28), is a complex of 28 kDa subunits that is thought to activate proteasomes toward the production of antigenic peptides. Human PSME1 and PSME2 genes encode the two proteasome activators PA28 alpha and beta, respectively, which have been implicated in antigen processing for loading class I MHC molecules. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"