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Product Name
PSMD5 antibody
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Description
PSMD5 Rabbit Polyclonal antibody. Positive IHC detected in human prostate cancer tissue. Positive WB detected in mouse liver tissue, human liver tissue, Jurkat cells. Positive IP detected in mouse liver tissue. Observed molecular weight by Western-blot: 50 kDa
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Tested applications
ELISA, IHC, IP, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
26S protease subunit S5 basic antibody; 26S proteasome subunit S5B antibody; KIAA0072 antibody; PSMD5 antibody; S5B antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PSMD5 recombinant protein (Accession Number: NM_005047). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:114243(PSMD5 antibody) at dilution of 1:100 (under 10x lens)
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:114243(PSMD5 Antibody) at dilution of 1:1000
IP Result of anti-PSMD5 (IP:Catalog No:114243, 4ug; Detection:Catalog No:114243 1:800) with mouse liver tissue lysate 4000ug.
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Background
PSMD5, , also named as KIAA0072, belongs to the proteasome subunit S5B/HSM3 family. It acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly, it is part of an intermediate PSMD5:PSMC2:PSMC1:PSMD2 module which probably assembles with a PSMD10:PSMC4:PSMC5:PAAF1 module followed by dissociation of PSMD5. (PMID:15489334). This antibody is specific to PSMD5.
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