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Product Name
PSMD13 antibody
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Description
PSMD13 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HepG2 cells, Jurkat cells. Positive IF detected in Hela cells. Observed molecular weight by Western-blot: 43 kDa
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Tested applications
ELISA, IF, WB
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Species reactivity
Human; other species not tested.
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Alternative names
HSPC027 antibody; p40.5 antibody; PSMD13 antibody; Rpn9 antibody; S11 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PSMD13 recombinant protein (Accession Number: NM_002817). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114396(PSMD13 Antibody) at dilution of 1:1000
Immunofluorescent analysis of Hela cells, using PSMD13 antibody Catalog No:114396 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
PSMD13 represents proteasome (prosome, macropain) 26S subunit, non-ATPase, 13
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References
- Li S, Izumi T, Hu J. Rescue of enzymatic function for disease-associated RPE65 proteins containing various missense mutations in non-active sites. The Journal of biological chemistry. 289(27):18943-56. 2014.
- Li S, Hu J, Jin RJ. Temperature-sensitive retinoid isomerase activity of RPE65 mutants associated with Leber Congenital Amaurosis. Journal of biochemistry. 158(2):115-25. 2015.
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