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PSMD11 antibody

Cat.#: 114394

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Product Information

  • Product Name
    PSMD11 antibody
  • Documents
  • Description
    PSMD11 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, A549 cells, MCF7 cells, mouse testis tissue, rat brain tissue. Positive IP detected in MCF-7 cells. Positive IF detected in MCF-7 cells. Observed molecular weight by Western-blot: 47 kDa
  • Tested applications
    ELISA, WB, IP, IF
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    p44.5 antibody; PSMD11 antibody; Rpn6 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of PSMD11 recombinant protein (Accession Number: NM_002815). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:1000-1:10000

    IP: 1:200-1:2000

    IF: 1:10-1:100

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114394(PSMD11 antibody) at dilution of 1:500

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114394(PSMD11 antibody) at dilution of 1:500

    IP Result of anti-PSMD11 (IP:Catalog No:114394, 3ug; Detection:Catalog No:114394 1:500) with MCF-7 cells lysate 2000ug.

    IP Result of anti-PSMD11 (IP:Catalog No:114394, 3ug; Detection:Catalog No:114394 1:500) with MCF-7 cells lysate 2000ug.

    Immunofluorescent analysis of MCF-7 cells using Catalog No:114394(PSMD11 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of MCF-7 cells using Catalog No:114394(PSMD11 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    The 26 S proteasome is a 2.5-MDa molecular machine that degrades ubiquitinated proteins in eukaryotic cells. It consists of a proteolytic core particle and two 19 S regulatory particles (RPs) composed of 6 ATPase (RPT) and 13 non-ATPase (RPN) subunits. PSMD11 gene encodes 19S proteasome subunit RPN6. Increased levels of PSMD11 and a corresponding increased assembly of the 26S/30S proteasome is correlated with high proteasome activity. In vitro ectopic expression of PSMD11 is sufficient to increase proteasome assembly and activity. Latest research has implicated PSMD11 in regulation of human embryonic stem cells function.
  • References
    • Qi T, Zhang W, Luan Y. Proteomic profiling identified multiple short-lived members of the central proteome as the direct targets of the addicted oncogenes in cancer cells. Molecular & cellular proteomics : MCP. 13(1):49-62. 2014.
    • Sparks A, Dayal S, Das J, Robertson P, Menendez S, Saville MK. The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a. Oncogene. 33(38):4685-96. 2014.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"