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Product Name
PSMC3 antibody
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Description
PSMC3 Rabbit Polyclonal antibody. Positive IHC detected in human ovary tumor tissue, human tonsillitis tissue. Positive IF detected in HEK-293 cells, HepG2 cells. Positive WB detected in HeLa cells, A431 cells, HEK-293 cells, HepG2 cells, Jurkat cells, MCF7 cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 49 kDa
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Tested applications
ELISA, WB, IF, IHC, IP
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Species reactivity
Human; other species not tested.
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Alternative names
Proteasome subunit P50 antibody; PSMC3 antibody; Tat binding protein 1 antibody; TBP 1 antibody; TBP1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PSMC3 recombinant protein (Accession Number: NM_002804). Purification method: Antigen Affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114390(PSMC3 antibody) at dilution of 1:1000
IP Result of anti-PSMC3 (IP:Catalog No:114390, 4ug; Detection:Catalog No:114390 1:800) with HeLa cells lysate 3200ug.
Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using Catalog No:114390(PSMC3 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using Catalog No:114390(PSMC3 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HEK-293 cells using Catalog No:114390(PSMC3 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
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Background
PSMC3(26S protease regulatory subunit 6A) is also named as TBP1, Proteasome 26S subunit ATPase 3 and belongs to the AAA ATPase family. The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. It is a multifunctional protein, component of the regulatory subunit of the proteasome, involved in different cellular processes.
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