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Product Name
PSMB3 antibody
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Description
PSMB3 Rabbit Polyclonal antibody. Positive IHC detected in human liver tissue, human brain tissue, human ovary tissue, human placenta tissue, human skin tissue, human testis tissue. Positive IF detected in HepG2 cells. Positive WB detected in HeLa cells, MCF7 cells. Observed molecular weight by Western-blot: 23kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
20S proteasome antibody; HC10 II antibody; Proteasome chain 13 antibody; Proteasome component C10 II antibody; Proteasome subunit beta type 3 antibody; Proteasome theta chain antibody; PSMB3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PSMB3 recombinant protein (Accession Number: NM_002795). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114383(PSMB3 antibody) at dilution of 1:200
Immunohistochemical of paraffin-embedded human liver using Catalog No:114383(PSMB3 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:114383(PSMB3 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:114383(PSMB3 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
PSMB3 represents proteasome (prosome, macropain) subunit, beta type, 3
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"