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Product Name
PSMA8 antibody
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Description
PSMA8 Rabbit Polyclonal antibody. Positive FC detected in HepG2 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human testis tissue. Positive WB detected in K-562 cells, Jurkat cells, mouse thymus tissue, RAW264.7 cells. Observed molecular weight by Western-blot: 28kd
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Tested applications
ELISA, WB, IHC, IF, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
PSMA7L antibody; PSMA8 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PSMA8 recombinant protein (Accession Number: NM_001025096). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:114378(PSMA8 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human testis using Catalog No:114378(PSMA8 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:114378(PSMA8 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using PSMA8 antibody Catalog No:114378 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
1X10^6 HepG2 cells were stained with .2ug PSMA8 antibody (Catalog No:114378, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
PSMA8 represents proteasome (prosome, macropain) subunit, alpha type, 8
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"