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Product Name
PSMA7 antibody
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Description
PSMA7 Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in HeLa cells, A431 cells, A549 cells, HepG2 cells, MCF7 cells, mouse brain tissue, Raji cells. Positive FC detected in HepG2 cells. Positive IF detected in HeLa cells. Positive IHC detected in human liver cancer tissue. Observed molecular weight by Western-blot: 28-30 kDa
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Tested applications
ELISA, WB, IHC, FC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
20S proteasome antibody; HSPC antibody; Proteasome subunit RC6 1 antibody; Proteasome subunit XAPC7 antibody; PSMA7 antibody; RC6 1 antibody; XAPC7 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PSMA7 recombinant protein (Accession Number: NM_002792). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114377(PSMA7 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:114377(PSMA7 antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:114377(PSMA7 antibody) at dilution of 1:50 (under 10x lens)
IP Result of anti-PSMA7 (IP:Catalog No:114377, 4ug; Detection:Catalog No:114377 1:500) with mouse brain tissue lysate 4000ug.
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:114377(PSMA7 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
1X10^6 HepG2 cells were stained with 0.2ug PSMA7 antibody (Catalog No:114377, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
PSMA7(Proteasome subunit alpha type-7) is also named as HSPC and belongs to the peptidase T1A family. It is an α-type subunit of the 20S proteasome core complex and participates in degrading proteins through ubiquitin-proteasome pathway (UPP) which plays an important role in the regulation of cell proliferation or cell cycle control, transcriptional regulation, immune and stress response, cell differentiation, and apoptosis.(PMID: 19442227).
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References
- Yang L, Tang Z, Zhang H. PSMA7 directly interacts with NOD1 and regulates its function. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology. 31(6):952-9. 2013.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"