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Product Name
PSMA6 antibody
- Documents
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Description
PSMA6 Rabbit Polyclonal antibody. Positive WB detected in MCF7 cells, A375 cells, A549 cells, HEK-293 cells, HepG2 cells, human brain tissue, human skeletal muscle tissue, Jurkat cells, Neuro-2a cells. Positive IP detected in HEK-293 cells. Positive IHC detected in human lung cancer tissue, human testis tissue. Positive IF detected in HeLa cells, A375 cells, HepG2 cells, MCF-7 cells. Observed molecular weight by Western-blot: 27-29 kDa
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Tested applications
ELISA, IHC, IF, WB, IP
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
20S proteasome antibody; 27 kDa prosomal protein antibody; IOTA antibody; Macropain iota chain antibody; p27K antibody; PROS 27 antibody; PROS27 antibody; Proteasome iota chain antibody; PSMA6 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PSMA6 recombinant protein (Accession Number: NM_002791). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:114376(PSMA6 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:114376(PSMA6 antibody) at dilution of 1:50 (under 10x lens)
IP Result of anti-PSMA6 (IP:Catalog No:114376, 4ug; Detection:Catalog No:114376 1:1000) with HEK-293 cells lysate 3200ug.
Immunofluorescent analysis of HeLa cells using Catalog No:114376(PSMA6 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
PSMA6(Proteasome subunit alpha type-6) is also named as PROS27 and belongs to the peptidase T1A family.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.It also has an ATP-dependent proteolytic activity.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"