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Product Name
PRUNE2 antibody
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Description
PRUNE2 Rabbit Polyclonal antibody. Positive WB detected in human placenta tissue, mouse brain tissue, mouse testis tissue, rat brain tissue, SH-SY5Y cells, Transfected HEK-293 cells. Positive IHC detected in human kidney tissue, human brain tissue, human prostate cancer tissue. Observed molecular weight by Western-blot: 140~150 kDa
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Tested applications
ELISA, IHC, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
A214N16.3 antibody; bA214N16.3 antibody; BMCC1 antibody; BNIPXL antibody; C9orf65 antibody; DKFZp762K117 antibody; FLJ50060 antibody; FLJ54876 antibody; FLJ59118 antibody; KIAA0367 antibody; Protein prune homolog 2 antibody; prune homolog 2 (Drosophila) antibody; PRUNE2 antibody; RP11 58J3.2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PRUNE2 recombinant protein (Accession Number: BC019095). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
Immunohistochemical of paraffin-embedded human kidney using Catalog No:114284(PRUNE2 antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:114284(PRUNE2 antibody) at dilution of 1:50 (under 40x lens)
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:114284(PRUNE2 Antibody) at dilution of 1:1000
human placenta tissue were subjected to SDS PAGE followed by western blot with Catalog No:114284(PRUNE2 Antibody) at dilution of 1:500
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Background
PRUNE2 gene, also named BMCC1, encodes protein prune homolog 2. PRUNE2 gene are overlapping with PCA3, one of the most prostate cancer specific markers. PRUNE2 may play an important role in regulating differentiation, survival and aggressiveness of the tumor cells. Besides its high level of expression seen in the nervous system (brain, cerebellum and spinal cord), it is also expressed at high levels in noneuroblastoma, rhabdomyosarcoma, melanoma and some osteosarcoma cell lines, whereas at only low levels in cancer cell lines of liver, breast, thyroid and colon. The Calculated molecular weight of this protein is 340 kDa. Our antibody specifically recognises the 140-150 kDa band which is an isoform or cleaved fragment of PRUNE2 (PMID:21234814).
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References
- Salameh A, Lee AK, Cardó-Vila M. PRUNE2 is a human prostate cancer suppressor regulated by the intronic long noncoding RNA PCA3. Proceedings of the National Academy of Sciences of the United States of America. 112(27):8403-8. 2015.
- Tatsumi Y, Takano R, Islam MS. BMCC1, which is an interacting partner of BCL2, attenuates AKT activity, accompanied by apoptosis. Cell death & disease. 6:e1607. 2015.
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