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Product Name
PRUNE antibody
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Description
PRUNE Rabbit Polyclonal antibody. Positive IHC detected in human liver cancer tissue. Positive IF detected in HepG2 cells. Positive IP detected in HepG2 cells. Positive WB detected in HepG2 cells, A431 cells, MCF7 cells. Observed molecular weight by Western-blot: 60kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DRES 17 antibody; DRES17 antibody; hPrune antibody; HTCD37 antibody; Protein prune homolog antibody; PRUNE antibody; prune homolog (Drosophila) antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PRUNE recombinant protein (Accession Number: NM_021222). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:114283(PRUNE antibody) at dilution of 1:600
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:114283(PRUNE antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using PRUNE antibody Catalog No:114283 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-PRUNE (IP:Catalog No:114283, 3ug; Detection:Catalog No:114283 1:500) with HepG2 cells lysate 1720ug.
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Background
PRUNE represents prune homolog (Drosophila)
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