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Product Name
PRPS1 antibody
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Description
PRPS1 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in Jurkat cells, HEK-293 cells, HeLa cells, mouse liver tissue, mouse spleen tissue, rat spleen tissue. Positive IF detected in HepG2 cells. Positive IHC detected in human testis tissue, human spleen tissue. Observed molecular weight by Western-blot: 35 kDa
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Tested applications
ELISA, IHC, IF, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ARTS antibody; CMTX5 antibody; KIAA0967 antibody; PPRibP antibody; PRPS1 antibody; PRS I antibody; PRSI antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PRPS1 recombinant protein (Accession Number: NM_002764). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:114237(PRPS1 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human testis using Catalog No:114237(PRPS1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:114237(PRPS1 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using PRPS1 antibody Catalog No:114237 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-PRPS1 (IP:Catalog No:114237, 4ug; Detection:Catalog No:114237 1:100) with HeLa cells lysate 2600ug.
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Background
PRPS1, also named as Ribose-phosphate pyrophosphokinase 1, is a 318 amino acid protein, which belongs to the ribose-phosphate pyrophosphokinase family. PRPS1 can form Homodimer and the active form is probably a hexamer composed of 3 homodimers. PRPS1 catalyzes the synthesis of phosphoribosylpyrophosphate (PRPP) that is essential for nucleotide synthesis. PRPS1 as a module has an association with the Hepatocellular carcinoma.
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References
- Bai C, Tang S, Bai C, Chen X. Quantitative proteomic dissection of a native 14-3-3ε interacting protein complex associated with hepatocellular carcinoma. Amino acids. 46(4):841-52. 2014.
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