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PRPF8 antibody

Cat.#: 114235

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Special Price 239.0 USD

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Product Information

  • Product Name
    PRPF8 antibody
  • Documents
  • Description
    PRPF8 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, human placenta tissue, Jurkat cells, K-562 cells. Observed molecular weight by Western-blot: 220 kDa
  • Tested applications
    ELISA, WB, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    HPRP8 antibody; p220 antibody; PRP8 antibody; PRP8 homolog antibody; PRPC8 antibody; PRPF8 antibody; RP13 antibody; Splicing factor Prp8 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of PRPF8 recombinant protein (Accession Number: NM_006445). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114235(PRPF8 antibody) at dilution of 1:800

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114235(PRPF8 antibody) at dilution of 1:800

    IP Result of anti-PRPF8 (IP:Catalog No:114235, 4ug; Detection:Catalog No:114235 1:500) with HeLa cells lysate 2000ug.

    IP Result of anti-PRPF8 (IP:Catalog No:114235, 4ug; Detection:Catalog No:114235 1:500) with HeLa cells lysate 2000ug.

  • Background
    PRPF8, a 220kd protein,is a component of mammalian spliceosomes, large multiprotein complexes involved in the removal of introns from mRNA precursor. It has a central structural role in the architecture of the U5 small nuclear ribonucleoprotein (snRNP) particle.
  • References
    • Coltri P, Effenberger K, Chalkley RJ, Burlingame AL, Jurica MS. Breaking up the C complex spliceosome shows stable association of proteins with the lariat intron intermediate. PloS one. 6(4):e19061. 2011.
    • Ilagan JO, Chalkley RJ, Burlingame AL, Jurica MS. Rearrangements within human spliceosomes captured after exon ligation. RNA (New York, N.Y.). 19(3):400-12. 2013.
    • Adler AS, McCleland ML, Yee S. An integrative analysis of colon cancer identifies an essential function for PRPF6 in tumor growth. Genes & development. 28(10):1068-84. 2014.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"