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Product Name
PRKCSH antibody
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Description
PRKCSH Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue. Positive IF detected in HeLa cells. Positive WB detected in HeLa cells, A431 cells, mouse liver tissue. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 80 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
80K H protein antibody; AGE R2 antibody; G19P1 antibody; Glucosidase 2 subunit beta antibody; Glucosidase II subunit beta antibody; PCLD antibody; PKCSH antibody; PLD1 antibody; PRKCSH antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PRKCSH recombinant protein (Accession Number: BC013586). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114200(PRKCSH antibody) at dilution of 1:1000
IP Result of anti-PRKCSH (IP:Catalog No:114200, 4ug; Detection:Catalog No:114200 1:1000) with HeLa cells lysate 2000ug.
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:114200(PRKCSH antibody) at dilution of 1:100 (under 10x lens)
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:114200(PRKCSH Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
PRKCSH ncodes the beta-subunit of glucosidase II, an N-linked glycan-processing enzyme in the endoplasmic reticulum (ER). This protein is an acidic phospho-protein known to be a substrate for protein kinase C. Defects in PRKCSH are a cause of an autosomal dominant polycystic liver disease (PCLD). Glucosidase II is an ER-localized enzyme that contains α and β subunits (Glucosidase IIα and Glucosidase IIβ) which form a defined heterodimeric complex.
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References
- Shin GC, Ahn SH, Choi HS. Hepatocystin/80K-H inhibits replication of hepatitis B virus through interaction with HBx protein in hepatoma cell. Biochimica et biophysica acta. 1832(10):1569-81. 2013.
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